MEMBRANE PRO INVITROGEN HOW TO

Open the Power Blotter Cassette by pressing the grey button on the lid. When using a PVDF membrane, be sure to wet the membrane with methanol or ethanol before soaking in 1X Power Blotter 1-Step Transfer Buffer. Four pieces of filter paper are required for each membrane. Use enough buffer to completely cover the filter papers and membrane. Before using, dilute to a 1X concentration using deionized water.Įquilibrate membrane and filter papers in the diluted Power Blotter 1-Step Transfer Buffer for a minimum of 5 minutes. The Power Blotter 1-Step Transfer Buffer comes in a 5X concentration. To begin the transfer process, flip the power switch at the rear of the device to turn on the system. The Power Blotter system has two different cassette sizes: a standard size for transferring one midi gel or up to 2 mini gels, and the XL cassette for transferring up to 2 midi gels or up to 4 mini gels at one time. In the Power Blotter System, the cathode and anode are plates that are fixed to the top and bottom of the cassette frame, and the transfer stack is placed in between them during the transfer process. The system can also be used for standard semi-dry transfer protocols that use traditional buffers. The Power Blotter 1-Step Transfer Buffer is an optimized, high-ionic strength buffer that facilitates rapid transfer conditions when combined with the high-current conditions of the Power Blotter System.įilter papers soaked in the 1-Step Transfer Buffer act as an ion reservoir.

MEMBRANE PRO INVITROGEN HOW TO

In this video, you’ll learn how to transfer proteins from polyacrylamide gels to membranes using the Invitrogen Power Blotter with our pre-cut membranes and filters, and Power Blotter 1-Step Transfer Buffer.

Membrane pro invitrogen